Nucleic-acid sequence based amplification in the rapid diagnosis of rabies.
Lancet
; 358(9285): 892-3, 2001 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-11567709
Current serological tests do not reliably diagnose rabies. We describe a technique based on amplification of nucleic-acid sequences to detect rabies-specific RNA in the saliva and cerebrospinal fluid (CSF) of four living patients with rabies. Rabies RNA could be detected in either saliva or CSF, or both, in all patients and as early as day 2 after onset of symptoms. Both saliva and CSF should be serially tested because not every sample can be expected to be positive. The whole process, including automated extraction, isothermal amplification, and detection can be done within 4 h.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rabia
/
Saliva
/
ARN Viral
/
Técnicas de Amplificación de Ácido Nucleico
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Lancet
Año:
2001
Tipo del documento:
Article
Pais de publicación:
Reino Unido