Thermodynamic stabilities of internal loops with GU closing pairs in RNA.
Biochemistry
; 40(38): 11509-17, 2001 Sep 25.
Article
en En
| MEDLINE
| ID: mdl-11560499
Many internal loops that form tertiary contacts in natural RNAs have GU closing pairs; examples include the tetraloop receptor and P1 helix docking site in group I introns. Thus, thermodynamic parameters of internal loops with GU closing pairs can contribute to the prediction of both secondary and tertiary structure. Oligoribonucleotide duplexes containing small internal loops with GU closing pairs were studied by optical melting, one-dimensional imino proton NMR, and one-dimensional phosphorus NMR. The thermodynamic stabilities of asymmetric internal loops with GU closing pairs relative to those of loops with GC closing pairs may be explained by hydrogen bonds. In contrast, the free energy increments for symmetric internal loops of two noncanonical pairs with GU closing pairs relative to loops with GC closing pairs show much more sequence dependence. Imino proton and phosphorus NMR spectra suggest that some GA pairs adjacent to GU closing pairs may form an overall thermodynamically stable but non-A-form conformation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligorribonucleótidos
/
Uracilo
/
ARN
/
Guanina
/
Conformación de Ácido Nucleico
Idioma:
En
Revista:
Biochemistry
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos