Suppression of CYP3A2 mRNA expression in the warfarin-resistant roof rat, Rattus rattus: possible involvement of cytochrome P450 in the warfarin resistance mechanism.
Xenobiotica
; 31(7): 399-407, 2001 Jul.
Article
en En
| MEDLINE
| ID: mdl-11531004
1. The continual use of warfarin as a rodenticide has caused the development of populations of warfarin-resistant roof rat. To study the biochemical mechanism of warfarin resistance, the mRNA expression levels of the major P450 forms in the warfarin-resistant and -susceptible roof rat liver following exposure to warfarin were quantified by competitive RT-PCR. 2. The constitutive levels of CYP2C11 and CYP3A2 mRNAs in the warfarin-resistant and -susceptible roof rat were extremely low compared with those in the STD rat. In response to warfarin administration, the CYP3A2 mRNA level in the warfarin-susceptible rat increased to about 3-fold of that before the treatment, whereas in the warfarin-resistant roof rat, CYP3A2 mRNA remained at a low level. 3. The present results suggest the possibility that reduced synthesis of CYP3A2 mRNA is involved in the warfarin-resistant mechanism in the roof rat.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rodenticidas
/
Esteroide Hidroxilasas
/
Warfarina
/
Resistencia a Medicamentos
/
ARN Mensajero
/
Hidrocarburo de Aril Hidroxilasas
/
Sistema Enzimático del Citocromo P-450
/
Esteroide 16-alfa-Hidroxilasa
Límite:
Animals
Idioma:
En
Revista:
Xenobiotica
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido