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Shah convolution differentiation Fourier transform for rear analysis in microchip capillary electrophoresis.
Kwok, Y C; Manz, A.
Afiliación
  • Kwok YC; AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology and Medicine, London, UK.
J Chromatogr A ; 924(1-2): 177-86, 2001 Jul 27.
Article en En | MEDLINE | ID: mdl-11521864
This paper first reports the application of Shah convolution differentiation Fourier transform for rear analysis. Rear analysis eliminates the need to create a well-defined and reproducible sample plug, thus making the operation simpler. The number of solution reservoirs, for microchip capillary electrophoresis (CE), could be reduced from the usual four to three. Sample bias in CE could be avoided too. The separation channel was first filled with the fluorescent sample solution, and subsequently flushed out with the buffer. The rear of each analyte zone gives rise to its flight of sigmoid-shaped steps in the time-domain. The time-domain detector signal was first differentiated and then Fourier transform was performed. The Fourier transform results were represented in the form of a magnitude plot. It is proposed that this would be as equally applicable to other separation techniques (e.g., chromatography) and detection methods (e.g., absorption).
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroforesis Capilar / Análisis de Fourier / Miniaturización Idioma: En Revista: J Chromatogr A Año: 2001 Tipo del documento: Article Pais de publicación: Países Bajos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroforesis Capilar / Análisis de Fourier / Miniaturización Idioma: En Revista: J Chromatogr A Año: 2001 Tipo del documento: Article Pais de publicación: Países Bajos