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Sphingolipid derivatives modulate intracellular Ca2+ in rat synaptosomes.
Miguel, B G; Calcerrada, M C; Catalán, R E; Martínez, A M.
Afiliación
  • Miguel BG; Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University, E-28040 Madrid, Spain.
Acta Neurobiol Exp (Wars) ; 61(2): 113-7, 2001.
Article en En | MEDLINE | ID: mdl-11512408
Sphingosylphosphorylcholine (SPC) induces a rapid increase of intracellular Ca2+ concentration in isolated synaptosomes. This effect is dose-dependent and is also dependent on extracellular Ca2+. Sphingosine (SPH) has a smaller effect and treatment with psychosine (PSY) is ineffective, which suggests that phosphorylation of the 1-carbon of SPH is required for the SPC to act as a Ca2+ release agonist in synaptosomes. Experiments performed in the presence of heparin or ryanodine indicate that SPC-elicited Ca2+ release is not mediated by IP3 or ryanodine receptors. Finally, our results show that the effect of SPC on Ca2+ concentration is nimodipine-sensitive, suggesting that SPC possibly activates a specific sphingolipid-gated Ca2+ channel in synaptosomes.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosforilcolina / Esfingolípidos / Esfingosina / Sinaptosomas / Calcio Límite: Animals Idioma: En Revista: Acta Neurobiol Exp (Wars) Año: 2001 Tipo del documento: Article País de afiliación: España Pais de publicación: Polonia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosforilcolina / Esfingolípidos / Esfingosina / Sinaptosomas / Calcio Límite: Animals Idioma: En Revista: Acta Neurobiol Exp (Wars) Año: 2001 Tipo del documento: Article País de afiliación: España Pais de publicación: Polonia