Changes of skeletal muscle proteases activities during a chronic low-frequency stimulation period.
Pflugers Arch
; 442(5): 745-51, 2001 Aug.
Article
en En
| MEDLINE
| ID: mdl-11512031
Modifications of muscular contractile patterns by chronic low-frequency stimulation induce structural, physiological, and biochemical transformations in fast-twitch fibers that cause them to act like slow-twitch muscle. During this transformation many changes in protein pattern appear and the proteolytic system may be involved in those changes. The activities of cathepsin L, B, H, D, the level of cystatin, as well as the calpain activity in rabbit fast-twitch muscle have been compared with those of slow-twitch muscle. The results show that fast-twitch muscle has lower cathepsin activities and higher calpain activities than slow-twitch muscle. Chronic low-frequency stimulation was applied for 24 days to fast-twitch muscles and changes in proteases and protease inhibitors (cystatin and calpastatin) were studied. After 7-14 days of stimulation, lysosomal cathepsin L, B, and D and cytoplasm calpain and proteosome activities increased several-fold. Involvement of the phagocyte cells in the protein fiber turnover was minimal. Although the turnover of contractile proteins during muscle electrostimulation takes place in synchrony with changes in the muscle proteolytic system, the stimulation period used did not attain the total transformation from fast- to slow-twitch muscle proteolytic pattern.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Calpaína
/
Catepsinas
/
Cistatinas
/
Fibras Musculares de Contracción Lenta
/
Fibras Musculares de Contracción Rápida
Límite:
Animals
Idioma:
En
Revista:
Pflugers Arch
Año:
2001
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Alemania