Detection of Vibrio cholerae 01 from aquatic environment in Sarawak.

Norazah, A; Zainuldin, M T; Kamel, A G; Kamaliah, M N; Taha, A M

Norazah, A; Zainuldin, M T; Kamel, A G; Kamaliah, M N; Taha, A M.

Afiliación

Norazah A; Bacteriology Division, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur.

Med J Malaysia ; 56(1): 4-9, 2001 Mar.

Article en En | MEDLINE | ID: mdl-11503295

RESUMEN

The detection of Vibrio cholerae 01 from the aquatic environment of Daro and Bintulu in Sarawak was carried out following an outbreak of cholera. Conventional culture methods and detection of ctx gene by polymerase chain reaction technique were carried out on 80 water samples. Only one sample was positive by culture methods while 8 were positive by PCR. DNA finger printing by pulsed-field gel electrophoresis showed that the clinical isolates in Daro and Bintulu were genetically identical while the environmental isolate was closely related. Recovery of Vibrio cholerae by culture method is poor and newer methods of detection should be developed.

Asunto(s)

Vibrio cholerae/aislamiento & purificación; Microbiología del Agua; Electroforesis en Gel de Campo Pulsado; Reacción en Cadena de la Polimerasa

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vibrio cholerae / Microbiología del Agua Tipo de estudio: Diagnostic_studies Idioma: En Revista: Med J Malaysia Año: 2001 Tipo del documento: Article Pais de publicación: Malasia

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