Incorporation of orotic acid into nucleotides and RNA in mouse organs during 60 minutes.
Hoppe Seylers Z Physiol Chem
; 356(4): 425-9, 1975 Apr.
Article
en En
| MEDLINE
| ID: mdl-1150156
Mouse kidney and liver were found to increase their levels of radioactivity above that of serum from 2 to 60 min after administration of [6-14C]orotic acid. In spleen, thymus and brain, the radioactivity level reached a maximum soon after the injection and then decreased, as did that in serum. Sixty minutes after the injection, 44% of the administered isotope dose was found in the kidneys, 22% in the liver and 0.75% in the spleen. The 14C activity in liver UTP increased rapidly and then remained constant for 60 min. The ratio between the activities in uridine phosphates and UDP-sugars was 3:4 from 10- 60 min after injection. In the liver and kidneys, the RNA 14C activities at 60 min after injection were 15% of the activity in their acid-soluble fractions. Intraperitoneal administration was found to be preferable to intravenous administration for studies on nucleotides and RNA in mouse liver, due to the delayed incorporation of the [14C]orotic acid activity into the nucleotide pool.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Orótico
/
Nucleótidos de Uracilo
/
ARN
Límite:
Animals
Idioma:
En
Revista:
Hoppe Seylers Z Physiol Chem
Año:
1975
Tipo del documento:
Article
Pais de publicación:
Alemania