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M-CSF transgenic mice: role of M-CSF in infection and autoimmunity.
Bernier, T; Halter, R; Pau, D; Rittinghausen, S; Emmendörffer, A.
Afiliación
  • Bernier T; Fraunhofer Institute of Toxicology and Aerosol Research, Department of Immunobiology, Hannover, Germany.
Exp Toxicol Pathol ; 53(2-3): 165-73, 2001 Jun.
Article en En | MEDLINE | ID: mdl-11484835
In this study, transgenic CD2F1 mouse lines (C-1.1-C-1.11) bearing a transgene encoding the murine growth factor M-CSF under the control of the liver specific alpha-1-antitrypsin gene promoter were generated. Transgenic C-1.4 mice showed elevated expression of transgene-encoded M-CSF in the liver and displayed a 2-3-fold increase of M-CSF plasma levels and of macrophage numbers in the liver as compared with non-transgenic littermates. M-CSF transgenic mice showed increased resistance against sublethal i.v. infections with Listeria monocytogenes as compared with infected non-transgenic mice. To investigate the influence of M-CSF in murine systemic lupus erythematosus (SLE), the M-CSF transgenic mouse line C-1.4 was bred into the genetic background of SLE-prone MRL+/+ mice. The resulting C-1.4/MRL transgenic mice bearing increased endogenous M-CSF levels showed consistently lower levels of anti-ss-DNA autoantibodies as compared with non-transgenic MRL+/+ mice. The life span of the C- 1.4/MRL transgenic mice and the severity of the disease in these mice remained unchanged as compared with their non-transgenic littermates. It is concluded that in addition to M-CSF further factors must be involved in the acceleration of the autoimmune disease in SLE prone MRL/lpr mice.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor Estimulante de Colonias de Macrófagos / Listeriosis / Lupus Eritematoso Sistémico Tipo de estudio: Prognostic_studies Aspecto: Patient_preference Límite: Animals Idioma: En Revista: Exp Toxicol Pathol Asunto de la revista: PATOLOGIA / TOXICOLOGIA Año: 2001 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor Estimulante de Colonias de Macrófagos / Listeriosis / Lupus Eritematoso Sistémico Tipo de estudio: Prognostic_studies Aspecto: Patient_preference Límite: Animals Idioma: En Revista: Exp Toxicol Pathol Asunto de la revista: PATOLOGIA / TOXICOLOGIA Año: 2001 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania