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CpG DNA induces cyclooxygenase-2 expression and prostaglandin production.
Chen, Y; Zhang, J; Moore, S A; Ballas, Z K; Portanova, J P; Krieg, A M; Berg, D J.
Afiliación
  • Chen Y; Department of Internal Medicine, University of Iowa College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, USA.
Int Immunol ; 13(8): 1013-20, 2001 Aug.
Article en En | MEDLINE | ID: mdl-11470771
Unmethylated CpG motifs found in bacterial DNA are potent activators of the innate and acquired immune systems, and rapidly induce the production of proinflammatory cytokines. We hypothesized that CpG DNA may also elicit the production of prostaglandins (PG), which are central lipid mediators of the immune and inflammatory response. To test our hypothesis, we stimulated murine spleen cells and RAW 264.7 murine macrophage cells with CpG DNA and assessed the effects on the PG synthesis pathway. Compared to control, DNA-containing CpG motifs induced >5-fold increase in PGE (2) production and rapidly up-regulated cyclooxygenase-2 (COX-2) at both the mRNA and protein level. CpG DNA was an extremely strong inducer of COX-2 as concentrations as low as 3 ng/ml induced COX-2 protein expression. The CpG DNA-induced PGE (2) down-regulated the immune response elicited by CpG. Blockade of PGE (2) production with selective COX-2 inhibitors or neutralizing anti-PGE (2) antibody markedly enhanced IFN-gamma secretion in vitro from CpG DNA-stimulated spleen cells. Moreover, selective COX-2 inhibition increased CpG DNA-induced IFN-gamma secretion in vivo. Inhibition of COX-2 also increased CpG DNA-induced lytic activity of NK cells. Taken together, these data indicate that DNA containing CpG motifs is a potent inducer of COX-2 and PGE (2) production. CpG-induced PG may subsequently down-regulate the immune and inflammatory responses elicited by the CpG DNA.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Prostaglandinas / Adyuvantes Inmunológicos / Prostaglandina-Endoperóxido Sintasas / Islas de CpG / Isoenzimas Límite: Animals Idioma: En Revista: Int Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Prostaglandinas / Adyuvantes Inmunológicos / Prostaglandina-Endoperóxido Sintasas / Islas de CpG / Isoenzimas Límite: Animals Idioma: En Revista: Int Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido