Identification of a system that allows a Rhizobium tropici dctA mutant to grow on succinate, but not on other C4-dicarboxylates.
Can J Microbiol
; 47(6): 509-18, 2001 Jun.
Article
en En
| MEDLINE
| ID: mdl-11467726
A defined insertion mutant of a gene encoding a homolog of the rhizobial C4-dicarboxylate permease (dctA) was constructed in Rhizobium tropici strain CIAT899. This mutant (GA1) was unable to grow on fumarate or malate; however, in contrast with other rhizobial dctA mutants, it retained a limited ability to grow on succinate with ammonia as a nitrogen source. Our results suggest the presence of a novel succinate-specific transport system in R. tropici. Biochemical characterization indicated that this alternative transport system in GAI is active and dependent on an energized membrane. It was also induced by succinate and aspartate, and was repressed by glucose and glycerol. Bean plants inoculated with GA1 showed a reduced nitrogen-fixing ability, achieving only 29% of the acetylene reduction activity determined in CIAT899 strain nodules, 33 days after inoculation. Also, bean plants inoculated with GA1 had reduced shoot dry weight compared with plants inoculated with the wild-type strain.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rhizobium
/
Proteínas Bacterianas
/
Ácido Succínico
/
Transportadores de Ácidos Dicarboxílicos
/
Ácidos Dicarboxílicos
/
Mutación
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Can J Microbiol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Uruguay
Pais de publicación:
Canadá