Irradiation enhances the support of haemopoietic cell transmigration, proliferation and differentiation by endothelial cells.

Gaugler, M H; Squiban, C; Mouthon, M A; Gourmelon, P; van der Meeren, A

Gaugler, M H; Squiban, C; Mouthon, M A; Gourmelon, P; van der Meeren, A.

Afiliación

Gaugler MH; Institut de Protection et de Sûreté Nucléaire, Département de Protection de la santé de l'Homme et de Dosimétrie, Section Autonome de Radiobiologie Appliquée à la Médecine, IPSN, Fontenay-aux-Roses, France. marie-helene.gaugler@ipsn.fr

Br J Haematol ; 113(4): 940-50, 2001 Jun.

Article en En | MEDLINE | ID: mdl-11442488

RESUMEN

Endothelial cells (ECs) are a critical component of the bone marrow stroma in the regulation of haemopoiesis. Recovery of bone marrow aplasia after radiation exposure depends, in part, on the repair of radiation-induced endothelial damage. Therefore, we assessed the ability of an irradiated human bone marrow EC line (TrHBMEC) to support transmigration, proliferation and differentiation of CD34+ bone marrow cells either irradiated or not in transendothelial migration or co-culture models. Radiation-induced EC damage was reflected by an increased release of soluble intercellular adhesion molecule (sICAM)-1 and platelet endothelial cell adhesion molecule (PECAM)-1. Irradiation of TrHBMECs with a 10 Gy dose strongly enhanced the transmigration of CD34+ cells, granulo-monocytic progenitors (CFU-GM) and erythroid progenitors (BFU-E). While ICAM-1 and PECAM-1 expression on irradiated TrHBMECs was increased, only antibodies against PECAM-1 inhibited the radiation-induced enhanced transmigration of haemopoietic cells. Irradiation of TrHBMECs (5-15 Gy) also increased proliferation and differentiation towards the granulo-monocytic lineage of co-cultured CD34+ cells, as well as colony formation by those cells and the production of interleukin 6 (IL-6), IL-8, granulocyte colony-stimulating factor (CSF) and granulocyte-macrophage CSF. Irradiated TrHBMECs were more capable of stimulating irradiated (1,2 Gy) CD34+ cells and haemopoietic progenitors than non-irradiated TrHBMECs. Together, these results suggest that, despite the radiation-induced damage, irradiated ECs may favour haemopoietic reconstitution after radiation exposure.

Asunto(s)

Células de la Médula Ósea/efectos de la radiación; Células Madre Hematopoyéticas/efectos de la radiación; Antígenos CD34; Antígenos CD13; Diferenciación Celular; División Celular; Línea Celular Transformada; Movimiento Celular; Técnicas de Cocultivo; Endotelio/citología; Endotelio/efectos de la radiación; Ensayo de Inmunoadsorción Enzimática/métodos; Factor Estimulante de Colonias de Granulocitos/metabolismo; Factor Estimulante de Colonias de Granulocitos y Macrófagos/metabolismo; Granulocitos; Células Madre Hematopoyéticas/citología; Células Madre Hematopoyéticas/inmunología; Humanos; Molécula 1 de Adhesión Intercelular/análisis; Interleucina-6/metabolismo; Interleucina-8/metabolismo; Antígeno de Macrófago-1; Microscopía de Contraste de Fase; Monocitos; Molécula-1 de Adhesión Celular Endotelial de Plaqueta/análisis; Complejo GPIIb-IIIa de Glicoproteína Plaquetaria; Complejo GPIb-IX de Glicoproteína Plaquetaria; Estadísticas no Paramétricas; Factores de Tiempo

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Células Madre Hematopoyéticas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Br J Haematol Año: 2001 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links