No evidence for binding between resistance gene product Cf-9 of tomato and avirulence gene product AVR9 of Cladosporium fulvum.

Luderer, R; Rivas, S; Nürnberger, T; Mattei, B; Van den Hooven, H W; Van der Hoorn, R A; Romeis, T; Wehrfritz, J M; Blume, B; Nennstiel, D; Zuidema, D; Vervoort, J; De Lorenzo, G; Jones, J D; De Wit, P J; Joosten, M H

Luderer, R; Rivas, S; Nürnberger, T; Mattei, B; Van den Hooven, H W; Van der Hoorn, R A; Romeis, T; Wehrfritz, J M; Blume, B; Nennstiel, D; Zuidema, D; Vervoort, J; De Lorenzo, G; Jones, J D; De Wit, P J; Joosten, M H.

Afiliación

Luderer R; Laboratory of Phytopathology, Wageningen University, The Netherlands.

Mol Plant Microbe Interact ; 14(7): 867-76, 2001 Jul.

Article en En | MEDLINE | ID: mdl-11437260

RESUMEN

The gene-for-gene model postulates that for every gene determining resistance in the host plant, there is a corresponding gene conditioning avirulence in the pathogen. On the basis of this relationship, products of resistance (R) genes and matching avirulence (Avr) genes are predicted to interact. Here, we report on binding studies between the R gene product Cf-9 of tomato and the Avr gene product AVR9 of the pathogenic fungus Cladosporium fulvum. Because a high-affinity binding site (HABS) for AVR9 is present in tomato lines, with or without the Cf-9 resistance gene, as well as in other solanaceous plants, the Cf-9 protein was produced in COS and insect cells in order to perform binding studies in the absence of the HABS. Binding studies with radio-labeled AVR9 were performed with Cf-9-producing COS and insect cells and with membrane preparations of such cells. Furthermore, the Cf-9 gene was introduced in tobacco, which is known to be able to produce a functional Cf-9 protein. Binding of AVR9 to Cf-9 protein produced in tobacco was studied employing surface plasmon resonance and surface-enhanced laser desorption and ionization. Specific binding between Cf-9 and AVR9 was not detected with any of the procedures. The implications of this observation are discussed.

Asunto(s)

Cladosporium/genética; Cladosporium/patogenicidad; Proteínas Fúngicas/genética; Genes Fúngicos; Genes de Plantas; Glicoproteínas de Membrana/genética; Proteínas de Plantas/genética; Solanum lycopersicum/genética; Solanum lycopersicum/microbiología; Animales; Células COS; Línea Celular; Proteínas Fúngicas/metabolismo; Solanum lycopersicum/metabolismo; Glicoproteínas de Membrana/metabolismo; Modelos Genéticos; Enfermedades de las Plantas/genética; Enfermedades de las Plantas/microbiología; Proteínas de Plantas/metabolismo; Plantas Modificadas Genéticamente; Unión Proteica; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Spodoptera; Resonancia por Plasmón de Superficie; Nicotiana/genética; Nicotiana/metabolismo; Virulencia/genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Proteínas Fúngicas / Glicoproteínas de Membrana / Cladosporium / Genes de Plantas / Solanum lycopersicum / Genes Fúngicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Plant Microbe Interact Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA / MICROBIOLOGIA Año: 2001 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos

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