Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography.
J Chromatogr B Biomed Sci Appl
; 757(1): 31-7, 2001 Jun 05.
Article
en En
| MEDLINE
| ID: mdl-11419746
A sensitive and reliable method was developed for the determination of hydrogen sulfide and acid-labile sulfur (ALS) in animal tissues using gas chromatography with flame photometric detector (GC-FPD) and ion chromatography (IC). Hydrogen sulfide trapped in alkaline solution was determined by GC-FPD as hydrogen sulfide or by IC as sulfate after oxidation with hydrogen peroxide. Sodium sulfide used as a source of hydrogen sulfide was standardized by IC. Fresh rat liver and heart tissues contained 112.2+/-23.0 and 274.1+/-34.6 nmol/g of ALS respectively. Free hydrogen sulfide was not detected.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Azufre
/
Cromatografía de Gases
/
Cromatografía Liquida
/
Sulfuro de Hidrógeno
/
Hígado
/
Miocardio
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos