Insulin gene expression pattern in rat pancreas during the estrous cycle.
Life Sci
; 68(26): 2979-85, 2001 May 18.
Article
en En
| MEDLINE
| ID: mdl-11411797
Sex steroid hormones influence insulin homeostasis and glucose metabolism, estradiol (E2) and progesterone (P4) induce changes in both fasting and postprandial insulinemia in rodents, however, insulin gene expression during estrous cycle is unknown. The aim of the present study was to determine an insulin gene expression pattern during the estrous cycle in the rat. Groups of 6 adult rats in each day of the estrous cycle were used. Serum P4, E2, testosterone (T) and insulin concentrations were determined by radioimmunoassay (RIA). A Northern blot analysis was performed to assess insulin gene expression in pancreatic tissue. We found a marked variation in insulin gene expression during the estrous cycle. The highest insulin expression was observed during the proestrus day. Interestingly, E2 and P4 but not T levels were correlated with changes in insulin mRNA content. The variations in serum insulin during the cycle were correlated with its mRNA content in pancreas. The overall results showed variations in serum insulin and insulin gene expression during estrous cycle of the rat that correlated with circulating E2 and P4 levels.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estro
/
Regulación de la Expresión Génica
/
Islotes Pancreáticos
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
Life Sci
Año:
2001
Tipo del documento:
Article
Pais de publicación:
Países Bajos