Antigen presentation using novel particulate organelles from halophilic archaea.
J Biotechnol
; 88(2): 119-28, 2001 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-11403846
A presentation vehicle was developed based on particulate gas vesicles produced by halophilic archaea. Gas vesicle epitope displays were prepared using standard coupling methods or recombinant DNA technology. When presented in the context of gas vesicle preparations, either the hapten, TNP, or a model six amino acid recombinant insert in the outer gas vesicle protein, GvpC was rendered immunogenic. Assays to quantify humoral responses indicated that each preparation elicited strong antibody responses in the absence of exogenous adjuvant. Thus, each preparation elicited a humoral response when injected into mice and this response was long lived and exhibited immunologic memory. Recombinant gas vesicle preparations therefore constitute a new, self-adjuvanting carrier/display vehicle for presentation of an array of peptidyl epitopes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Orgánulos
/
Proteínas
/
Proteínas Arqueales
/
Halobacterium
/
Memoria Inmunológica
/
Proteínas de la Membrana
/
Antígenos
Límite:
Animals
Idioma:
En
Revista:
J Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Países Bajos