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Cellular localization of monoamine oxidase A and B in human tissues outside of the central nervous system.
Rodríguez, M J; Saura, J; Billett, E E; Finch, C C; Mahy, N.
Afiliación
  • Rodríguez MJ; Unitat de Bioquímica, Institut d'Investigacions Biomédiques August Pi i Sunye, Facultat de Medicina, Universitat de Barcelona, Spain.
Cell Tissue Res ; 304(2): 215-20, 2001 May.
Article en En | MEDLINE | ID: mdl-11396715
We studied the localization of monoamine oxidase (MAO) A and B in human heart, liver, duodenum, blood vessels and kidney by immunohistochemistry. The primary antibodies used were mouse monoclonal anti-human MAO-A (6G11/E1) and anti-human MAO-B (3F12/G10/2E3). Samples were obtained from six routine autopsy cases and fixed in 2% paraformaldehyde. All cardiomyocytes and hepatocytes showed MAO-A and MAO-B immunoreactivity. In the duodenum, both immunoreactivities were present in all cells of the villi, Lieberkühn crypts, muscularis mucosae and muscular layers, whereas Brunner glands were devoid of MAO-A and MAO-B staining. Endothelial cells of lymphatic vessels showed MAO-A but no MAO-B immunoreactivity, whereas arteries and veins presented MAO-A and MAO-B staining in muscular layers and fibroblasts but not in endothelial cells. In the kidney, renal tubuli showed MAO-A and MAO-B immunoreactivities, whereas collecting ducts and the Bowman's capsule showed only MAO-A staining. These data represent the first study of the cellular distribution of MAO-A and MAO-B in these human tissues. They show that both enzymes have a widespread distribution in the human body with a matching pattern in many, but not all tissues, and with strong differences from the pattern of distribution in rodents.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Monoaminooxidasa Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Tissue Res Año: 2001 Tipo del documento: Article País de afiliación: España Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Monoaminooxidasa Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Tissue Res Año: 2001 Tipo del documento: Article País de afiliación: España Pais de publicación: Alemania