Bioelectric recognition assay (BERA).

Kintzios, S; Pistola, E; Panagiotopoulos, P; Bomsel, M; Alexandropoulos, N; Bem, F; Ekonomou, G; Biselis, J; Levin, R

Kintzios, S; Pistola, E; Panagiotopoulos, P; Bomsel, M; Alexandropoulos, N; Bem, F; Ekonomou, G; Biselis, J; Levin, R.

Afiliación

Kintzios S; Laboratory of Plant Physiology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece. spirokintzios@matrix.kapatel.gr

Biosens Bioelectron ; 16(4-5): 325-36, 2001 Jun.

Article en En | MEDLINE | ID: mdl-11390221

RESUMEN

A novel biosensory method has been developed for the determination of various chemical and biological molecules by assessing their electrophysiological interactions with a group of cells and cell components immobilized in a gel matrix that preserves their 'physiological' functions. The method was applied for the detection of: (i) hepatitis C virus in human blood samples; (ii) plant viruses; and (iii) a herbicide (glyphosate) in aqueous solutions. It was able to rapidly (assay time 3-5 min) and specifically detect the molecules in question at a concentration lower than 100 pg/ml, among other compounds f similar structure. The potential use of BERA biosensors for a rapid and cost-efficient molecule determination without prior knowledge of a specific receptor-molecule interaction is discussed.

Asunto(s)

Técnicas Biosensibles/métodos; Animales; Supervivencia Celular; Células Inmovilizadas; Humanos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles Límite: Animals / Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2001 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Reino Unido

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