Lung transplant reperfusion injury involves pulmonary macrophages and circulating leukocytes in a biphasic response.

Fiser, S M; Tribble, C G; Long, S M; Kaza, A K; Cope, J T; Laubach, V E; Kern, J A; Kron, I L

Fiser, S M; Tribble, C G; Long, S M; Kaza, A K; Cope, J T; Laubach, V E; Kern, J A; Kron, I L.

Afiliación

Fiser SM; Department of Thoracic and Cardiovascular Surgery, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA. smf9e@virginia.edu

J Thorac Cardiovasc Surg ; 121(6): 1069-75, 2001 Jun.

Article en En | MEDLINE | ID: mdl-11385373

RESUMEN

OBJECTIVE: Both donor pulmonary macrophages and recipient circulating leukocytes may be involved in reperfusion injury after lung transplantation. By using the macrophage inhibitor gadolinium chloride and leukocyte filters, we attempted to identify the roles of these two populations of cells in lung transplant reperfusion injury. METHODS: With our isolated, ventilated, blood-perfused rabbit lung model, all groups underwent lung harvest followed by 18-hour cold storage and 2-hour blood reperfusion. Measurements of pulmonary artery pressure, lung compliance, and arterial oxygenation were obtained. Group I (n = 8) served as a control. Group II (n = 8) received gadolinium chloride at 14 mg/kg 24 hours before lung harvest. Group III (n = 8) received leukocyte-depleted blood reperfusion by means of a leukocyte filter. RESULTS: The gadolinium chloride group had significantly improved arterial oxygenation and pulmonary artery pressure measurements compared with control subjects and an improved arterial oxygenation compared with the filter group after 30 minutes of reperfusion. After 120 minutes of reperfusion, however, the filter group had significantly improved arterial oxygenation and pulmonary artery pressure measurements compared with the control group and an improved arterial oxygenation compared with the gadolinium chloride group. CONCLUSIONS: Lung transplant reperfusion injury occurs in two phases. The early phase is mediated by donor pulmonary macrophages and is followed by a late injury induced by recipient circulating leukocytes.

Asunto(s)

Leucocitos/fisiología; Trasplante de Pulmón/efectos adversos; Trasplante de Pulmón/fisiología; Macrófagos/fisiología; Daño por Reperfusión/etiología; Daño por Reperfusión/fisiopatología; Análisis de Varianza; Animales; Modelos Animales de Enfermedad; Femenino; Gadolinio/farmacología; Supervivencia de Injerto; Recuento de Leucocitos; Leucocitos/efectos de los fármacos; Pulmón/irrigación sanguínea; Pulmón/patología; Pulmón/fisiopatología; Rendimiento Pulmonar; Trasplante de Pulmón/métodos; Macrófagos/efectos de los fármacos; Masculino; Filtros Microporos; Tamaño de los Órganos; Oxígeno/sangre; Conejos; Valores de Referencia; Sensibilidad y Especificidad; Recolección de Tejidos y Órganos/métodos; Resistencia Vascular

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Trasplante de Pulmón / Leucocitos / Macrófagos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Thorac Cardiovasc Surg Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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