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Identification of epistatic interactions involved in non-insulin-dependent diabetes mellitus in the Otsuka Long-Evans Tokushima Fatty rat.
Yamada, T; Miyake, T; Sugiura, K; Narita, A; Wei, K; Wei, S; Moralejo, D H; Ogino, T; Gaillard, C; Sasaki, Y; Matsumoto, K.
Afiliación
  • Yamada T; Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Kyoto 606-8502.
Exp Anim ; 50(2): 115-23, 2001 Apr.
Article en En | MEDLINE | ID: mdl-11381614
The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is an animal model for obese-type non-insulin-dependent diabetes mellitus (NIDDM) in humans. Our present investigation was designed to identify epistatic interactions influencing NIDDM by performing least squares analysis of variance of all pairs of informative markers in 160 F2 progenies bred from an intercross of OLETF and Fischer-344 rats. We identified four interactions between Nidd15/of (chromosome 7) and Nidd16/of (chromosome 14), Nidd15/of and Nidd17/of (chromosome 15), Nidd16/of and Nidd18/of (chromosome 15), and Nidd16/of and Nidd19/of (chromosome 17), which account for a total of approximately 40% of the genetic variation of entire glucose levels after glucose challenge in the F2. The Nidd16/of locus, which is involved in three of four digenic interactions, and the Nidd19/of are likely to correspond to Nidd2/of and Nidd14/of, NIDDM loci previously identified in the F2 by single-QTL model and multiple-QTL model, respectively, while Nidd15/of, Nidd17/of and Nidd18/of loci reflect novel NIDDM loci. An aberrant increase of the entire glucose level due to synergism occurs in the double OLETF homozygote genotype of Nidd15/of and Nidd16/of, and of Nidd16/of and Nidd19/of, as well as in the OLETF homozygote genotypes of Nidd15/of and Nidd16/of, respectively, combined with the heterozygote genotypes of Nidd17/of and Nidd18/of. These findings demonstrate that inter-allelic interactions are likely to be an important component of NIDDM susceptibility.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diabetes Mellitus / Diabetes Mellitus Tipo 2 / Epistasis Genética / Obesidad Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Exp Anim Asunto de la revista: MEDICINA VETERINARIA Año: 2001 Tipo del documento: Article Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diabetes Mellitus / Diabetes Mellitus Tipo 2 / Epistasis Genética / Obesidad Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Exp Anim Asunto de la revista: MEDICINA VETERINARIA Año: 2001 Tipo del documento: Article Pais de publicación: Japón