[Intrathecal injection of NOS antisense oligonucleotides inhibits the increase of NMDA1AR mRNA expression in the spinal cord and brainstem of morphine-withdrawal rats].
Sheng Li Xue Bao
; 53(1): 27-31, 2001 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-11354793
Methods of reverse transcription polymerase chain reaction (RT-PCR), intrathecal injection and antisense drugs were used to study the effects of nitric oxide (NO) on the scores of morphine-withdrawal syndrome and the expression of NMDA1AR mRNA in rat spinal cord and brainstem. Intrathecal injection of NOS antisense oligonucleotides (AS-ONs) significantly decreased the scores of morphine-withdrawal symptoms. The effect of nNOS AS-ONs was greater than that of eNOS AS-ONs. The expression of NMDA1AR mRNA in the spinal cord and brainstem increased in morphine-dependent rats and increased to a greater extent in morphine-withdrawal rats. Intrathecal injection of nNOS AS-ONs significantly inhibited the increased expression of NMDA1AR mRNA in the spinal cord and brainstem of morphine-withdrawal rats. Intrathecal injection of eNOS antisense oligonucleotides inhibited the expression of NMDA1AR mRNA in the spinal cord of morphine-withdrawal rats, but did not in the brainstem. It is suggested that NO mediates morphine-withdrawal reaction and participates in modulating the expression of NMDA1AR mRNA in morphine-withdrawal rats.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Médula Espinal
/
Síndrome de Abstinencia a Sustancias
/
Tronco Encefálico
/
Oligonucleótidos Antisentido
/
Receptores de N-Metil-D-Aspartato
/
Óxido Nítrico Sintasa
/
Morfina
Límite:
Animals
Idioma:
Zh
Revista:
Sheng Li Xue Bao
Año:
2001
Tipo del documento:
Article
Pais de publicación:
China