Carbohydrate composition of Carcinus aestuarii hemocyanin.
Arch Biochem Biophys
; 389(2): 153-8, 2001 May 15.
Article
en En
| MEDLINE
| ID: mdl-11339803
The hemocyanin of the crab Carcinus aestuarii contains a carbohydrate moiety that represents 1.6% of protein mass. This carbohydrate content is higher than that exhibited by other arthropod hemocyanins so far investigated. By combination of FPLC ion exchange chromatography and reverse-phase HPLC, the native oligomeric protein can be resolved into three major and one minor electrophoretically pure fractions that are found to be homogeneous by N-terminal sequencing and correspond to the subunit polypeptide chains. Sugar analysis on the different subunits reveals that the subunit referred to as Ca2 is glycosylated, with a carbohydrate content of 6.3%. By Ca2 trypsin digestion, separation of glycopeptides, and amino acid sequencing, three consensus sequences for O-glycosylation and one for N-glycosylation were found. MALDI-MS was applied for the determination of the molecular masses of the various glycopeptides and peptides after removal of carbohydrates by neuraminidase and alpha-N-acetylgalactosaminidase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hemocianinas
/
Braquiuros
Límite:
Animals
Idioma:
En
Revista:
Arch Biochem Biophys
Año:
2001
Tipo del documento:
Article
País de afiliación:
Bulgaria
Pais de publicación:
Estados Unidos