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N-tert-butyl-alpha-phenylnitrone, a free radical scavenger with anticholinesterase activity does not improve the cognitive performance of scopolamine-challenged rats.
Milivojevic, N; Babic, K; Milatovic, D; Dettbarn, W D; Sket, D; Zivin, M.
Afiliación
  • Milivojevic N; Brain Research Laboratory, Institute of Pathophysiology, Medical School, University of Ljubljana, Zaloska 4, 1000, Ljubljana, Slovenia.
Int J Dev Neurosci ; 19(3): 319-25, 2001 Jun.
Article en En | MEDLINE | ID: mdl-11337201
Reversible inhibitors of acetylcholinesterase improve spatial learning and memory in animal models of cognitive impairment. Here we investigate if the beneficial effects of free radical scavenger N-tert-butyl-alpha-phenylnitrone (PBN) on cognitive performance could be explained by its recently discovered anticholinesterase activity. Morris water maze experiment was performed to examine the effect of PBN on the impairment of spatial learning and memory induced by the antagonist of cholinergic muscarinic transmission scopolamine. In situ hybridization histochemistry experiment was performed to study its effects on the induction of immediate early gene expression (c-fos, c-jun) by dopamine D1 receptor agonist SKF-82958 and on the augmentation of the SKF-82958-induced expression of these genes by scopolamine. In both experiments, the effects of PBN were compared to the effects of reversible anticholinesterase physostigmine. We found that physostigmine but not PBN significantly reversed the cognitive impairment in scopolamine-challenged rats, prevented the induction of c-fos and c-jun mRNAs by SKF-82958 and attenuated the augmentation of the SKF-82958-induced expression of these genes by scopolamine. The present experiments did not reveal a significant in vivo anticholinesterase activity of PBN.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escopolamina / Inhibidores de la Colinesterasa / Depuradores de Radicales Libres / Cognición / Antagonistas Muscarínicos / Óxidos de Nitrógeno Límite: Animals Idioma: En Revista: Int J Dev Neurosci Año: 2001 Tipo del documento: Article País de afiliación: Eslovenia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escopolamina / Inhibidores de la Colinesterasa / Depuradores de Radicales Libres / Cognición / Antagonistas Muscarínicos / Óxidos de Nitrógeno Límite: Animals Idioma: En Revista: Int J Dev Neurosci Año: 2001 Tipo del documento: Article País de afiliación: Eslovenia Pais de publicación: Estados Unidos