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Isolation and characterization of rhesus blood dendritic cells using flow cytometry.
Pichyangkul, S; Saengkrai, P; Yongvanitchit, K; Limsomwong, C; Gettayacamin, M; Walsh, D S; Stewart, V A; Ballou, W R; Heppner, D G.
Afiliación
  • Pichyangkul S; Department of Immunology and Medicine, US Army Medical Component, US Armed Forces Research Institute of Medical Science (AFRIMS), 315/6 Rajvithi Road, 10400, Bangkok, Thailand. SathitP@THAI.AMEDD.ARMY.MIL
J Immunol Methods ; 252(1-2): 15-23, 2001 Jun 01.
Article en En | MEDLINE | ID: mdl-11334961
Recognition of dendritic cells (DCs) as initiators and modulators of immune responses and growing use of rhesus monkeys for the preclinical optimization of vaccine formulations prompted characterization of the phenotype and function of isolated rhesus peripheral blood DCs. We developed a flow cytometric method to directly identify and isolate DCs from rhesus peripheral blood whereby a T cell depleted population negative for CD3, CD14, CD16 and CD20 but positive for CD83 yielded a cell population with surface markers, morphology, and a cytokine profile similar to human myeloid DCs. Rhesus blood DCs were more effective than monocytes and B cells in mixed lymphocyte reactions and in the presentation of recombinant malaria blood stage antigen MSP-1((42)) to autologous T cells. The ability to isolate rhesus blood DC from peripheral blood should be a useful tool for immunological investigations.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Separación Celular / Citometría de Flujo Límite: Animals / Humans Idioma: En Revista: J Immunol Methods Año: 2001 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Separación Celular / Citometría de Flujo Límite: Animals / Humans Idioma: En Revista: J Immunol Methods Año: 2001 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos