CCK expression in enteroendocrine cell is regulated by soluble factor(s) from underlying fibroblasts.
Mol Cell Endocrinol
; 175(1-2): 5-13, 2001 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-11325512
Studies on the cross-talk between the intestinal epithelium and the underlying connective tissue have concentrated on enterocytes. In contrast, little is known about the interactions between the mesenchymal compartment and the enteroendocrine cells, scattered among the other cell types of the epithelium. To address this question, a panel of coculture systems between the enteroendocrine STC-1 cell line and three intestinal myofibroblastic cell lines (MIC) was used in order to assess different levels of regulation, namely cell-cell and cell-matrix interactions, and the role of diffusible factors. We demonstrate that the expression of cholecystokinin, a typical intestinal hormone produced by STC-1 cells, is up-regulated in the presence of a fibroblastic environment through a paracrine pathway involving FGF2. Concomitantly, STC-1 cell morphology and proliferation were also modulated, but through distinct mechanisms according to the origin of fibroblasts. The results reveal definite epithelio-mesenchymal interactions that may be critical for the maintenance of phenotype and function of enteroendocrine cells.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colecistoquinina
/
Células Enteroendocrinas
/
Fibroblastos
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Endocrinol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Irlanda