Serum induces a transcriptional activation of CYP1A1 gene in HepG2 independently of the AhR pathway.
Life Sci
; 68(18): 2141-50, 2001 Mar 23.
Article
en En
| MEDLINE
| ID: mdl-11324719
CYP1A1 is largely implicated in carcinogenesis. To date, it is known that this gene is induced by xenobiotics such as polycyclic aromatic hydrocarbons. In this study, we evaluated the effect of serum in the regulation of CYP1A1 gene expression. CYP1A1 mRNA level is induced 1) in HepG2 and HT29-D4 cells by 3-methylcholanthrene 2) only in HepG2 after treatment by serum. The CYP1A1 mRNA induction in HepG2 is the consequence at least in part of a transcriptional activation as was demonstrated by evaluation of the hnRNA level. HepG2 cells were transfected by a plasmid containing the 7.5 Kb of the CYP1A1 promoter and the CAT reporter gene. No CAT stimulation was observed after serum treatment. These results demonstrated that CYP1A1 is induced at a transcriptional level by a physiological compound contained in serum independently of the Ah receptor and the 7.5 Kb promoter region.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sangre
/
Albúmina Sérica Bovina
/
Células Tumorales Cultivadas
/
Receptores de Hidrocarburo de Aril
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Carcinoma Hepatocelular
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Citocromo P-450 CYP1A1
/
Neoplasias Hepáticas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Life Sci
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Países Bajos