Isolation and characterization of the Enterobacter cloacae cheR mutant defective in phosphate taxis.
Biosci Biotechnol Biochem
; 65(2): 456-8, 2001 Feb.
Article
en En
| MEDLINE
| ID: mdl-11302189
A chemotaxis-defective mutant of Enterobacter cloacae IFO3320, designated EC1, was isolated after N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. Computer-assisted capillary assays showed that EC1 failed to show chemotactic responses to peptone and inorganic phosphate (Pi). Cloning and sequence analysis showed that EC1 is a cheR mutant, suggesting that Pi taxis by E. cloacae is dependent on a methyl-accepting chemotaxis protein(s) (MCP). EC1 was further mutagenized with NTG to construct cheR pstS and cheR pstA double mutants. A recombinant plasmid pECT01.2, which contained the E. cloacae cheR gene, restored the ability of these double mutants to show chemotaxis toward peptone but not Pi. These results suggest that the phosphate-specific transport (Pst) system, together with a MCP(s), is required for detecting Pi in E. cloacae.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Quimiotaxis
/
Enterobacter cloacae
/
Metiltransferasas
/
Mutación
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido