Demonstration in yeast of the function of BP-80, a putative plant vacuolar sorting receptor.
Plant Cell
; 13(4): 781-92, 2001 Apr.
Article
en En
| MEDLINE
| ID: mdl-11283336
BP-80, later renamed VSR(PS-1), is a putative receptor involved in sorting proteins such as proaleurain to the lytic vacuole, with its N-terminal domain recognizing the vacuolar sorting determinant. Although all VSR(PS-1) characteristics and in vitro binding properties described so far favored its receptor function, this function remained to be demonstrated. Here, we used green fluorescent protein (GFP) as a reporter in a yeast mutant strain defective for its own vacuolar receptor, Vps10p. By expressing VSR(PS-1) together with GFP fused to the vacuolar sorting determinant of petunia proaleurain, we were able to efficiently redirect the reporter to the yeast vacuole. VSR(PS-1) is ineffective on GFP either alone or when fused with another type of plant vacuolar sorting determinant from a chitinase. The plant VSR(PS-1) therefore interacts specifically with the proaleurain vacuolar sorting determinant in vivo, and this interaction leads to the transport of the reporter protein through the yeast secretory pathway to the vacuole. This finding demonstrates VSR(PS-1) receptor function but also emphasizes the differences in the spectrum of ligands between Vps10p and its plant equivalent.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Plantas
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Vacuolas
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Levaduras
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Receptores de Superficie Celular
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Proteínas de Transporte Vesicular
Idioma:
En
Revista:
Plant Cell
Asunto de la revista:
BOTANICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Reino Unido