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Neuroprotective effects of estrogen against beta-amyloid toxicity are mediated by estrogen receptors in cultured neuronal cells.
Kim, H; Bang, O Y; Jung, M W; Ha, S D; Hong, H S; Huh, K; Kim, S U; Mook-Jung, I.
Afiliación
  • Kim H; Brain Disease Research Center, Ajou University School of Medicine, 442-721, Suwon, South Korea.
Neurosci Lett ; 302(1): 58-62, 2001 Apr 13.
Article en En | MEDLINE | ID: mdl-11278111
Although estrogen is known to exert beneficial effects on Alzheimer's disease, its underlying cellular mechanisms have not been clear. In this study we investigated whether or not neuroprotective effects of estrogen are mediated by estrogen receptors (ERs). Treatment of estrogen (1.8 nM) reduced beta-amyloid (Abeta)-induced death of ER-expressing W4 cells. This effect of estrogen was blocked by a specific ER blocker ICI 182,780. When estrogen was treated to HT22 cells, which lack functional ERs, Abeta-induced cell death was not affected. Transfection of HT22 cells with human ERalpha, but not ERbeta, restored protective action of estrogen against Abeta. Hoechst staining revealed that estrogen protected ERalpha-expressing cells by blocking Abeta-induced apoptosis. These results indicate that estrogen blocks Abeta-induced cell death via ERalpha-dependent pathways.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Estrógenos / Péptidos beta-Amiloides / Apoptosis / Estradiol / Estrógenos / Neuronas Límite: Humans Idioma: En Revista: Neurosci Lett Año: 2001 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Irlanda
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Estrógenos / Péptidos beta-Amiloides / Apoptosis / Estradiol / Estrógenos / Neuronas Límite: Humans Idioma: En Revista: Neurosci Lett Año: 2001 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Irlanda