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IL-12-mediated increases in protection elicited by pneumococcal and meningococcal conjugate vaccines.
Buchanan, R M; Briles, D E; Arulanandam, B P; Westerink, M A; Raeder, R H; Metzger, D W.
Afiliación
  • Buchanan RM; Department of Microbiology and Immunology, Medical College of Ohio, Toledo 43614, USA.
Vaccine ; 19(15-16): 2020-8, 2001 Feb 28.
Article en En | MEDLINE | ID: mdl-11228373
Interleukin-12 (IL-12) may be a beneficial adjuvant for augmenting vaccine efficacy against encapsulated bacteria such as Streptococcus pneumoniae and Neisseria meningitidis since it can stimulate production of interferon-gamma (IFN-gamma) and secretion of antibody isotypes that are efficient at mediating complement fixation and opsonophagocytosis. In this study, we demonstrate the ability of IL-12 to enhance murine antibody responses, particularly IgG2a levels, to both pneumococcal and meningococcal conjugate vaccines. Transfer of immune serum from mice immunized with the meningococcal conjugate vaccine and IL-12 resulted in increased survival times, whereas transfer of serum from mice immunized with the pneumococcal conjugate and IL-12 resulted in protection from death upon bacterial challenge. Although treatment with vaccine and IL-12 increased levels of IFN-gamma mRNA, IL-12-mediated enhancement of antibody responses still occurred in IFN-gamma(-/-) mice. The results demonstrate the effectiveness of IL-12 as an adjuvant for polysaccharide conjugate vaccines, especially the pneumococcal conjugate vaccine.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interleucina-12 / Vacunas Meningococicas / Vacunas Neumococicas Límite: Animals Idioma: En Revista: Vaccine Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interleucina-12 / Vacunas Meningococicas / Vacunas Neumococicas Límite: Animals Idioma: En Revista: Vaccine Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos