Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma Brief report.

Yu, Q; Lu, Y; Nerurkar, V R; Yanagihara, R

Yu, Q; Lu, Y; Nerurkar, V R; Yanagihara, R.

Afiliación

Yu Q; Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu 96816, USA.

Arch Virol ; 145(12): 2669-76, 2000.

Article en En | MEDLINE | ID: mdl-11205112

RESUMEN

A 1,632-bp fragment, flanking the original 483-bp region of the DNA polymerase gene of a novel herpesvirus found in tissues of green turtles (Chelonia mydas) with fibropapilloma, was amplified from the circularized EcoRI-cut DNA extracted from tumor tissues by inverse PCR. The resultant 2,019-bp partial sequence of the DNA polymerase gene of the newfound herpesvirus, including the original 483-bp region, showed a high degree of homology at both the nucleotide and amino acid levels with that of other human and animal herpesviruses. Phylogenetic analysis confirmed that this novel herpesvirus belonged to the Alphaherpesvirinae subfamily.

Asunto(s)

Alphaherpesvirinae/genética; Genes Virales; Infecciones por Herpesviridae/veterinaria; Papiloma/veterinaria; Neoplasias Cutáneas/veterinaria; Tortugas/virología; Alphaherpesvirinae/clasificación; Alphaherpesvirinae/enzimología; Animales; Secuencia de Bases; Clonación Molecular; ADN Viral/análisis; ADN Polimerasa Dirigida por ADN/genética; Datos de Secuencia Molecular; Filogenia; Reacción en Cadena de la Polimerasa

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papiloma / Neoplasias Cutáneas / Tortugas / Alphaherpesvirinae / Infecciones por Herpesviridae / Genes Virales Límite: Animals Idioma: En Revista: Arch Virol Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Austria

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