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Clonidine attenuates the carbachol-induced contractile and phosphatidylinositol responses of rat trachea.
Shibata, O; Saito, M; Hashimoto, S; Sakai, K; Makita, T; Sumikawa, K.
Afiliación
  • Shibata O; Department of Anesthesiology, Nagasaki University School of Medicine, Japan. opshiba@net.nagasaki-u.ac.jp
J Pharm Pharmacol ; 52(12): 1523-8, 2000 Dec.
Article en En | MEDLINE | ID: mdl-11197082
Although clonidine is known to affect vascular smooth muscle, its effects on airway smooth muscle are not fully understood. This study was designed to examine the effects of clonidine on carbachol-induced contractile and phosphatidylinositol responses of rat trachea. Clonidine, at a dose of 100 microM or greater, attenuated carbachol-induced contraction and the accumulation of carbachol-induced inositol monophosphate (IP1). Clonidine also attenuated the accumulation of aluminium fluoride-induced IP1. The concentration-effect relationship of IP1 accumulation was similar to that of carbachol-induced contraction; r = 0.797, P < 0.001. These results suggest that clonidine attenuates contractile responses, at least in part, through the inhibition of phospholipase C (coupled with G-proteins) in phosphatidylinositol responses.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilinositoles / Tráquea / Carbacol / Clonidina / Analgésicos / Contracción Muscular Límite: Animals Idioma: En Revista: J Pharm Pharmacol Año: 2000 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilinositoles / Tráquea / Carbacol / Clonidina / Analgésicos / Contracción Muscular Límite: Animals Idioma: En Revista: J Pharm Pharmacol Año: 2000 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido