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Tumor necrosis factor-alpha but not interleukin-1 beta or interleukin-8 concentrations correlate with angiogenic activity of peritoneal fluid from patients with minimal to mild endometriosis.
Maas, J W; Calhaz-Jorge, C; ter Riet, G; Dunselman, G A; de Goeij, A F; Struijker-Boudier, H A.
Afiliación
  • Maas JW; Research Institute Growth and Development (GROW), Department of Obstetrics and Gynaecology, Academisch Ziekenhuis Maastricht, Maastricht, The Netherlands. jmaa@sgyn.azm.nl
Fertil Steril ; 75(1): 180-5, 2001 Jan.
Article en En | MEDLINE | ID: mdl-11163835
OBJECTIVE: To assess the angiogenic activity of peritoneal fluid in women with minimal to mild endometriosis and to investigate the relationship between this activity and the concentration of macrophage-derived angiogenic factors and clinical variables, such as phase of menstrual cycle, type of lesion, and revised American Society for Reproductive Medicine classification. DESIGN: In vivo bioassay. SETTING: Tertiary-care university medical center. PATIENT(S): Fifty-two female volunteers with laparoscopic findings indicating minimal to mild endometriosis. INTERVENTION(S): Peritoneal fluid was collected at the start of laparoscopy. A standard amount of peritoneal fluid was applied to a chick embryo chorioallantoic membrane assay. MAIN OUTCOME MEASURE(S): Angiogenic response was assessed by determining the vascular density index. RESULT(S): 85% of the peritoneal fluid samples induced angiogenesis in the chick embryo chorioallantoic membrane bioassay. Tumor necrosis factor-alpha and total protein were significantly related to the vascular density index, whereas interleukin-1beta, interleukin-8, and clinical variables appeared to not affect the angiogenic response. CONCLUSION(S): The results confirms previous findings of peritoneal fluid angiogenic activity in women with minimal to mild endometriosis and indicate involvement of tumor necrosis factor-alpha.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquido Ascítico / Interleucina-8 / Interleucina-1 / Factor de Necrosis Tumoral alfa / Endometriosis / Neovascularización Patológica Tipo de estudio: Diagnostic_studies Límite: Adult / Animals / Female / Humans Idioma: En Revista: Fertil Steril Año: 2001 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Líquido Ascítico / Interleucina-8 / Interleucina-1 / Factor de Necrosis Tumoral alfa / Endometriosis / Neovascularización Patológica Tipo de estudio: Diagnostic_studies Límite: Adult / Animals / Female / Humans Idioma: En Revista: Fertil Steril Año: 2001 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos