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NPS 1506: a novel NMDA receptor antagonist: neuroprotective effects in a model of closed head trauma in rats.
Lorber, A; Artru, A A; Lam, M A; Mueller, L A; Karpas, Z; Roytblat, L; Shapira, Y.
Afiliación
  • Lorber A; Department of Analytical Chemistry, Nuclear Research Center of the Negev, Beer Sheva, Israel.
J Neurosurg Anesthesiol ; 12(4): 345-55, 2000 Oct.
Article en En | MEDLINE | ID: mdl-11147383
We examined whether NPS 1506, a novel uncompetitive N-methyl-D-aspartate receptor antagonist, influences neurological outcome following closed head trauma (CHT) in rats. One hundred ten rats were divided into 11 groups: CHT (yes/no), treatment with NPS 1506 (yes/no), and time of euthanization (24 h/48 h). The dose of NPS 1506 was 1 mg/kg IV at 1 and 4 hours following CHT or sham operation. Closed head trauma induced the following changes in the injured hemisphere: Decreased specific gravity (sg) (1.036 +/- 0.006) and magnesium (Mg) (0.042 +/- 0.005 microg/mg) at 24 hours, and potassium (K) at 24 (1.145 +/- 0.376 microg/mg) and 48 hours, and increased water content (W) (84.9 +/- 2.5%) and sodium (Na) (2.135 +/- 0.699 microg/mg) at 24 hours, and calcium (Ca) at 24 (0.543 +/- 0.157 microg/mg) and 48 hours. These were reversed by NPS 1506; sg of 1.043 +/- 0.004, Mg of 0.077 +/- 0.009 microg/mg, K of 1.930 +/- 0.238 microg/mg, W of 81.5 +/- 1.9%, Ca of 0.043 +/- 0.023 microg/mg, and Na of 0.688 +/- 0.110 microg/mg. In groups not given NPS 1506, a nonsignificant decrease in neurological severity score (NSS) occurred at 24 and 48 hours as compared to NSS at 1 hour after CHT. In groups given NPS 1506, NSS at 24 and 48 hours decreased significantly (improved) compared to NSS at 1 hour, but not compared to NSS at 24 and 48 hours in groups not given NPS 1506. NPS 1506 caused no significant change in ischemic tissue volume or hemorrhagic necrosis volume in the injured hemisphere at 24 hours or 48 hours. These findings indicate that NPS 1506 improved measures of brain tissue edema (at 24 hours but not at 48 hours) and ion homeostasis, and this improvement was not related to other measures of outcome.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos Cerrados de la Cabeza / Receptores de N-Metil-D-Aspartato / Fármacos Neuroprotectores / Fluorobencenos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Neurosurg Anesthesiol Asunto de la revista: ANESTESIOLOGIA / NEUROCIRURGIA Año: 2000 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos Cerrados de la Cabeza / Receptores de N-Metil-D-Aspartato / Fármacos Neuroprotectores / Fluorobencenos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Neurosurg Anesthesiol Asunto de la revista: ANESTESIOLOGIA / NEUROCIRURGIA Año: 2000 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos