An organically modified silicate-based ethanol biosensor.
Anal Biochem
; 288(1): 39-43, 2001 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-11141304
A novel electrocatalytic ethanol biosensor using ferrocene-encapsulated palladium (Pd)-linked organically modified sol-gel glass (ormosil) is reported. The alkoxy precursors used to prepare the new ormosil-based electrocatalytic biosensor are Pd-linked glycidoxypropyltrimethoxysilane and trimethoxysilane. Pd-glycidoxypropyltrimethoxysilane (black solution) is made by mixing aqueous solutions of palladium chloride and glycidoxypropyltrimethoxysilane. The new ormosil is made using a Pd-linked silane precursor, trimethoxysilane, an aqueous solution of ferrocene monocarboxylic acid, and HCl. Alcohol dehydrogenase (ADH) is assembled over the ferrocene-ormosil layer using polyvinyl alcohol and then protecting the immobilized enzyme layer using Millipore filter membranes (pore size 1 microm). The electrocatalytic response of immobilized ADH, soluble nicotinamide adenine dinucleotide, and Pd-linked ormosil-encapsulated ferrocene is then observed. The electrocatalytic oxidation of NADH and the subsequent ADH-catalyzed formation of NADH are monitored electrochemically. Typical results recorded after the addition of varying concentrations of ethanol are reported; however, the sensor is sensitive to other alcohol and known ADH-sensitive substrates. The stability and reproducibility of the new ethanol biosensor are reported.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Silanos
/
Técnicas Biosensibles
/
Etanol
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2001
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Estados Unidos