Expression of the melanin-concentrating hormone receptor in porcine and human ciliary epithelial cells.

Hintermann, E; Erb, C; Talke-Messerer, C; Liu, R; Tanner, H; Flammer, J; Eberle, A N

Hintermann, E; Erb, C; Talke-Messerer, C; Liu, R; Tanner, H; Flammer, J; Eberle, A N.

Afiliación

Hintermann E; Laboratory of Endocrinology, Department of Research, University Eye Clinic, University Hospital and University Children's Hospital, Basel, Switzerland.

Invest Ophthalmol Vis Sci ; 42(1): 206-9, 2001 Jan.

Article en En | MEDLINE | ID: mdl-11133869

RESUMEN

PURPOSE: To evaluate whether the receptors for melanin-concentrating hormone (MCH) and its functional antagonist alpha-melanocyte-stimulating hormone (alpha-MSH) are expressed in the ciliary epithelium. Furthermore, to examine whether MCH, a neuropeptide involved in fluid and electrolyte homeostasis, may influence ion flux mediated by Na,K (adenosine triphosphatase)-ATPase in a ciliary epithelial cell line. METHODS: Expression of MCH receptors (MCH-R) and alpha-MSH receptors (MSH-R) on primary porcine ciliary pigmented epithelial (PE) cells and on a human nonpigmented ciliary epithelial (NPE) cell line, ODM-2 was investigated by radioligand binding studies and reverse transcription-polymerase chain reaction (RT-PCR). The MCH-R was further characterized by photocrosslinking. Influence of MCH on Na, K-ATPase activity was evaluated by an Rb(+) transport assay. RESULTS: MCH-R expression was observed at both the mRNA and protein levels in PE and NPE cells. In contrast, MSH-Rs were not detectable. At the mRNA level, expression of slc-1 was shown and with crosslinking, a 44-kDa protein was labeled. MCH showed no effect on Na,K-ATPase activity of NPE cells. CONCLUSIONS: The presence of MCH-R in ciliary epithelial cells of both human and porcine origin but the absence of MSH-Rs indicates that in these cells, MCH and alpha-MSH do not form a functionally antagonistic hormonal pair as they do in several other systems. Although effects of MCH on intestinal water and ion transport have been documented, a direct control of Na,K-ATPase activity was not detected in human NPE cells in vitro.

Asunto(s)

Cuerpo Ciliar/metabolismo; Proteínas del Ojo/genética; Epitelio Pigmentado Ocular/metabolismo; ARN Mensajero/biosíntesis; Receptores de la Hormona Hipofisaria/genética; Animales; Unión Competitiva; Línea Celular; Cartilla de ADN/química; Proteínas del Ojo/biosíntesis; Expresión Génica; Proteínas de Unión al GTP Heterotriméricas/metabolismo; Humanos; Ensayo de Unión Radioligante; Receptores de la Hormona Hipofisaria/biosíntesis; Receptores de Somatostatina/metabolismo; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa; ATPasa Intercambiadora de Sodio-Potasio/metabolismo; Porcinos; alfa-MSH/biosíntesis; alfa-MSH/genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Epitelio Pigmentado Ocular / Receptores de la Hormona Hipofisaria / ARN Mensajero / Cuerpo Ciliar / Proteínas del Ojo Límite: Animals / Humans Idioma: En Revista: Invest Ophthalmol Vis Sci Año: 2001 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos

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