Studies on the mechanism of orthophosphate regulation of bovine brain hexokinase.
J Biol Chem
; 250(5): 1864-71, 1975 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-1112835
An attempt was made to gain insight into the mechanism of orthophosphate attenuation of glucose-6-P inhibition of bovine brain hexokinase I (ADP:D-hexose 6-phosphotransferase, EC 2.7.1.1) from experiments of ligand binding and initial rate kinetics. Studies of glucose-6-P and phosphate binding to hexokinase reveal one binding site per hexokinase molecule. A model is presented which is consistent with the binding and kinetic data currently available on the alleviation of glucose-6-P inhibition of brain hexokinase by phosphate. The model implies that hexokinase may exist in equilibrium either as a free or phosphate-associated enzyme. The kinetic parameters of the two enzyme forms are similar except in their ability to bind glucose-6-P. It is suggested that the dissociation constant for glucose-6-P is relatively very high for hexokinase to which phosphate is bound. Phosphate appears to bind at an allosteric site on the enzyme, whereas glucose-6-P is associated either at the active site or at an allosteric site which overlaps the catalytic site.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatos
/
Encéfalo
/
Hexoquinasa
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
1975
Tipo del documento:
Article
Pais de publicación:
Estados Unidos