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Induction of CD95 ligand expression on T lymphocytes and B lymphocytes and its contribution to apoptosis of CD95-up-regulated CD4+ T lymphocytes in macaques by infection with a pathogenic simian/human immunodeficiency virus.
Sasaki, Y; Ami, Y; Nakasone, T; Shinohara, K; Takahashi, E; Ando, S; Someya, K; Suzaki, Y; Honda, M.
Afiliación
  • Sasaki Y; Department of Safety Research on Biologics, National Institute of Infectious Diseases, Tokyo, Japan. yuko@nih.go.jp
Clin Exp Immunol ; 122(3): 381-9, 2000 Dec.
Article en En | MEDLINE | ID: mdl-11122244
Using an established SIV/HIV-C2/1-infected cynomolgus monkey model displaying stable CD4+ T cell depletion, the kinetics of apoptosis and the levels of expression of CD95 membrane-associated CD95L on lymphocytes were investigated to test the involvement of the CD95/CD95L system in CD4+ T lymphocyte loss in vivo. Rapid depletion of CD4+ T cells occurred up to 2 weeks after infection, with chronic CD4+ T lymphopenia thereafter. During the initial CD4+ T cell loss, which was accompanied by viraemia, about 90% of the peripheral CD4+ T cell subset underwent spontaneous apoptotic cell death during 24 h of culture. Increased expression of CD95 was observed on both CD4+ and CD8+ T cell subsets, with CD95 expression on CD8+ cells declining rapidly, but high CD95 expression being maintained on CD4+ cells. Since CD95L was expressed on CD8+ T cells, B cells and to a lesser extent on CD4+ T cells, this suggests that CD95-mediated apoptosis might be controlled in an autocrine/paracrine fashion.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Linfocitos B / Linfocitos T CD4-Positivos / Infecciones por VIH / Regulación hacia Arriba / VIH / Virus de la Inmunodeficiencia de los Simios / Apoptosis / Receptor fas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Clin Exp Immunol Año: 2000 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Linfocitos B / Linfocitos T CD4-Positivos / Infecciones por VIH / Regulación hacia Arriba / VIH / Virus de la Inmunodeficiencia de los Simios / Apoptosis / Receptor fas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Clin Exp Immunol Año: 2000 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido