Genomic diversity and prevalence of Rotavirus in cow and buffalo calves in northern India.
Rev Sci Tech
; 19(3): 871-6, 2000 Dec.
Article
en En
| MEDLINE
| ID: mdl-11107633
Faecal samples were collected from seventy-eight diarrhoeic cow and buffalo calves between November 1998 and February 1999 to study the genomic diversity and prevalence of Rotavirus infection by ribonucleic acid polyacrylamide gel electrophoresis (RNA-PAGE) and enzyme-linked immunosorbent assay (ELISA). In the organised dairy farm (where daily production and health records were maintained), the overall prevalence of infection with Rotavirus, recorded by RNA-PAGE and ELISA, was 27.02% (10/37) in both cow and buffalo calves. In unorganised dairy herds (where no production or health records were maintained), RNA-PAGE and ELISA detected infection with Rotavirus in 26.8% (11/41) of cow and 19.5% (8/41) of buffalo calves. Five distinct electropherotypes were found to circulate in cow and buffalo calves. All were short electropherotypes except the single long electropherotype observed in a buffalo calf in an unorganised dairy herd. Some differences in RNA migration pattern were observed when these electropherotypes were compared with the neonatal calf diarrhoea virus strain of Rotavirus. Some electropherotypes were restricted to one farm while others were found in both organised and unorganised dairy herds and in both cow and buffalo calves.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones por Rotavirus
/
Variación Genética
/
Búfalos
/
Enfermedades de los Bovinos
/
Rotavirus
/
Diarrea
Tipo de estudio:
Prevalence_studies
/
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Rev Sci Tech
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MEDICINA VETERINARIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Francia