Application of liquid chromatography-nuclear magnetic resonance spectroscopy to the identification of natural products.
J Chromatogr B Biomed Sci Appl
; 748(1): 259-67, 2000 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-11092603
LC-NMR combines the separation power of high-performance liquid chromatography (HPLC) with the superior structural information content of nuclear magnetic resonance (NMR). These two techniques traditionally have been the primary tools used by natural products chemists to isolate and determine the structures of molecules of interest. Recent advances in NMR technology have allowed the practical application of LC-NMR, thus providing natural products chemists with a hyphenated technique which combines the two most important tools in their field. A brief review of the literature describing how LC-NMR has been applied to natural products research is followed by a specific example illustrating how this technique was used to identify the marine alkaloid aaptamine (1). Aaptamine was identified as the active component in the crude dichloromethane extract of the sponge Aaptos sp. which had been determined to possess inhibitory activity against the enzyme glutamine:fructose-6-phosphate amidotransferase (GFAT) by a high throughput screening (HTS) effort. Isolated aaptamine (1) exhibited an IC50 = 120 microM against this enzyme. The experience gained from the identification of aaptamine was used to define a strategy for the use of LC-NMR in a natural products HTS program.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Productos Biológicos
/
Espectroscopía de Resonancia Magnética
/
Cromatografía Líquida de Alta Presión
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Países Bajos