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Expression of TAFII70 RNA and protein during oogenesis and development of the amphibian Pleurodeles waltl.
Giani, L; Bucci, S; Ragghianti, M; Mancino, G.
Afiliación
  • Giani L; Dipartimento di Fisiologia e Biochimica, Laboratorio di Biologia cellulare e dello sviluppo, Università degli Studi di Pisa, Pisa, Italy.
Mech Dev ; 99(1-2): 191-4, 2000 Dec.
Article en En | MEDLINE | ID: mdl-11091092
TAFs are thought to play an essential role in eukaryotic RNA polymerase II transcription by mediating the expression of distinct subsets of genes. TAFII60/70 was studied in yeast, Drosophila and humans: in the present work, we analyzed the homologue PwTAFII70 in Pleurodeles. The gene is expressed in ovarian oocytes and throughout development, and the level of expression decreases in late embryos. The transcripts are localized in the animal hemisphere of the fertilized eggs and in the animal blastomeres of embryos at cleavage; later PwTAFII70 mRNA is expressed in the neural plate and folds. TAFII70 protein, which is present in fertilized eggs and throughout development, progressively shows a lower level of expression starting from the neurula stage.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Oogénesis / Pleurodeles / Factores de Transcripción Límite: Animals Idioma: En Revista: Mech Dev Asunto de la revista: EMBRIOLOGIA Año: 2000 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Irlanda
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Oogénesis / Pleurodeles / Factores de Transcripción Límite: Animals Idioma: En Revista: Mech Dev Asunto de la revista: EMBRIOLOGIA Año: 2000 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Irlanda