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Identification of an Arabidopsis thaliana mutant accumulating threonine resulting from mutation in a new dihydrodipicolinate synthase gene.
Sarrobert, C; Thibaud, M C; Contard-David, P; Gineste, S; Bechtold, N; Robaglia, C; Nussaume, L.
Afiliación
  • Sarrobert C; Laboratoire du Métabolisme Carboné, UMR 163, CNRS-CEA, DSV.DEVM, CEA/Cadarache, F-13108 St Paul les Durance Cedex, France.
Plant J ; 24(3): 357-67, 2000 Nov.
Article en En | MEDLINE | ID: mdl-11069709
A novel Arabidopsis DHDPS gene named DHDPS2 was found through identification of a mutant by promoter trapping. The mutation promotes a reduction of growth resulting from combination of a defect in lysine biosynthesis and accumulation of a toxic level of threonine or derived products. The mutant also modifies the amino acid composition issuing from the pyruvate and aspartate pathways, affecting mainly the root compartment. These data are in accordance with the expression of DHDPS2 in the root apex as visualized by expression of the GUS reporter gene. This suggests that a large proportion of the amino acids derived from pyruvate and aspartate are synthesized in this organ.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Treonina / Arabidopsis / Hidroliasas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2000 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Treonina / Arabidopsis / Hidroliasas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2000 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido