The presence and specificity of crustacean cardioactive peptide (CCAP)-immunoreactivity in gastropod neurons.

Hernádi, L; Agricola, H J

Hernádi, L; Agricola, H J.

Afiliación

Hernádi L; Department of Experimental Zoology, Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany. hernadi@tres.blki.hu

Acta Biol Hung ; 51(2-4): 147-52, 2000.

Article en En | MEDLINE | ID: mdl-11034139

RESUMEN

CCAP-like immunoreactivity was detected in central neurons with small and medium diameters in both Helix and Lymnaea CNS. The intensity of immunoreactivity showed seasonal changes with a maximum intensity during spring. The overwhelming majority of nerve cell bodies exhibiting CCAP immunoreactivity is located in the cerebral and parietal ganglia of both Helix and Lymnaea. The neurons of pleural and buccal ganglia were devoid of CCAP-immunoreactivity. Following preabsorbtion of CCAP antibody in 1:15000 dilution with 10(-3) M CCAP or CCAP-related peptide (Helix -CCAP), immunoreactivity could not be observed in neurons, demonstrating the specificity of the antibody to CCAP-related molecules in both Helix and Lymnaea.

Asunto(s)

Caracoles Helix/metabolismo; Lymnaea/metabolismo; Neuropéptidos/metabolismo; Secuencia de Aminoácidos; Animales; Sistema Nervioso Central/metabolismo; Inmunohistoquímica; Neuronas/metabolismo; Neuropéptidos/química

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neuropéptidos / Caracoles Helix / Lymnaea Límite: Animals Idioma: En Revista: Acta Biol Hung Año: 2000 Tipo del documento: Article Pais de publicación: Hungria

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