Effect of combined treatment with perindoprilat and low-power red light laser irradiation on human erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity.
Scand J Clin Lab Invest
; 60(5): 395-402, 2000 Aug.
Article
en En
| MEDLINE
| ID: mdl-11003259
Erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity were estimated after in vitro combined treatment of human erythrocytes with perindoprilat and low-power red light irradiation. Membrane fluidity was determined using fluorescent labels spectroscopy; membrane potential was evaluated by means of potential-sensitive fluorescent dyes; and acetylcholinesterase activity was estimated using the Ellman method. Both perindoprilat and laser irradiation, when used separately, increase microviscosity in the polar region and hyperpolarize the membranes in comparison with control erythrocytes. The combined action of these agents does not cause any further change in these parameters. Perindoprilat has an additional inhibitory effect on the activity of acetylcholinesterase, whereas laser irradiation causes an increase in the activity of the enzyme. Their combined action restores the initial activity of the enzyme independently of the sequence of treatment with both agents.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilcolinesterasa
/
Eritrocitos
/
Indoles
/
Rayos Láser
/
Fluidez de la Membrana
/
Potenciales de la Membrana
Límite:
Humans
Idioma:
En
Revista:
Scand J Clin Lab Invest
Año:
2000
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Reino Unido