Unfolding forces of titin and fibronectin domains directly measured by AFM.
Adv Exp Med Biol
; 481: 129-36; discussion 137-41, 2000.
Article
en En
| MEDLINE
| ID: mdl-10987070
AFM-based Single Molecule Force Spectroscopy provides a new tool for probing the mechanical properties of single molecules. In this chapter we show that the unfolding forces of single protein domains can be directly measured. Unfolding forces give new insight into protein stability that cannot be deduced from thermodynamic measurements. A comparison between the unfolding forces measured in Ig domains of the muscle protein titin and those measured in fibronectin Type III domains reveals an extraordinarily high stability of titin domains.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Quinasas
/
Fibronectinas
/
Proteínas Musculares
Idioma:
En
Revista:
Adv Exp Med Biol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos