Early detection of Leishmania promastigotes in dog bone marrow cultures by acridine orange stain.

Barreca, G S; Matera, G; De Majo, M; Lamberti, A; Liberto, M C; Focà, A

Barreca, G S; Matera, G; De Majo, M; Lamberti, A; Liberto, M C; Focà, A.

Afiliación

Barreca GS; Department of Medical Sciences, Institute of Microbiology, University of Catanzaro, 88100, Catanzaro, Italy.

Diagn Microbiol Infect Dis ; 37(4): 247-51, 2000 Aug.

Article en En | MEDLINE | ID: mdl-10974575

RESUMEN

An acridine orange staining technique was evaluated in comparison with other well-known methods for the laboratory diagnosis of leishmaniasis. A higher number of promastigotes was found in Novy-MacNeal-Nicolle (NNN) cultures inoculated with canine bone marrow, when culture samples were stained with acridine orange vital stain, compared with those detected using either Giemsa staining or unstained wet mount examination. Based on our data the acridine orange stain is a useful and timely technique in reflecting the true numbers of microorganisms present in a culture and also enhances the visualization of the parasites. The present results warrant further studies with human samples from suspected leishmaniasis patients.

Asunto(s)

Células de la Médula Ósea/parasitología; Leishmania; Leishmaniasis/diagnóstico; Naranja de Acridina; Animales; Células de la Médula Ósea/citología; Técnicas de Cultivo de Célula; Perros; Colorantes Fluorescentes; Sensibilidad y Especificidad; Coloración y Etiquetado

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Leishmaniasis / Leishmania Tipo de estudio: Diagnostic_studies / Evaluation_studies / Screening_studies Límite: Animals Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2000 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links