Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography.

Quiñones-Torrelo, C; Martín-Biosca, Y; Sagrado, S; Villanueva-Camañas, R M; Medina-Hernández, M J

Quiñones-Torrelo, C; Martín-Biosca, Y; Sagrado, S; Villanueva-Camañas, R M; Medina-Hernández, M J.

Afiliación

Quiñones-Torrelo C; Departamento de Química Analítica, Facultad de Farmacia, Universidad de Valencia, C/Vicente Andrés Estellés s/n, E-46100 Burjassot, Valencia, Spain.

Biomed Chromatogr ; 14(5): 287-92, 2000 Aug.

Article en En | MEDLINE | ID: mdl-10960826

RESUMEN

A new liquid chromatographic procedure for the determination of amobarbital and secobarbital in plasma samples is proposed. The method uses a Spherisorb octadecylsilane ODS-2 C(18) analytical column, a guard column of similar characteristics and 0.04 M CTAB solution buffered at pH 7.5 containing 3% 1-propanol as micellar mobile phase. The UV detection was carried out at 250 nm. Butabarbital was used as internal standard. Plasma samples preparation only required adequate dilution with the mobile phase before injection into the chromatographic system. The limits of detection were 0.2 and 0.4 mg/L for amobarbital and secobarbital, respectively. The proposed method allows the determination of amobarbital and secobarbital in plasma at therapeutic levels.

Asunto(s)

Amobarbital/sangre; Cromatografía Liquida/métodos; Hipnóticos y Sedantes/sangre; Secobarbital/sangre; Humanos; Sensibilidad y Especificidad; Espectrofotometría Ultravioleta

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Secobarbital / Cromatografía Liquida / Amobarbital / Hipnóticos y Sedantes Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Biomed Chromatogr Año: 2000 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links