Myosin-V stepping kinetics: a molecular model for processivity.
Proc Natl Acad Sci U S A
; 97(17): 9482-6, 2000 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-10944217
Myosin-V is a molecular motor that moves processively along its actin track. We have used a feedback-enhanced optical trap to examine the stepping kinetics of this movement. By analyzing the distribution of time periods separating discrete approximately 36-nm mechanical steps, we characterize the number and duration of rate-limiting biochemical transitions preceding each such step. These data show that myosin-V is a tightly coupled motor whose cycle time is limited by ADP release. On the basis of these results, we propose a model for myosin-V processivity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Unión a Calmodulina
/
Proteínas Motoras Moleculares
/
Miosina Tipo V
/
Modelos Biológicos
/
Movimiento
/
Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos