Differences in regulation of pH(i) in large (>/=10 nuclei) and small (
Am J Physiol Cell Physiol
; 279(3): C751-61, 2000 Sep.
Article
en En
| MEDLINE
| ID: mdl-10942726
Osteoclasts are multinucleated cells that resorb bone by extrusion of protons and proteolytic enzymes. They display marked heterogeneity in cell size, shape, and resorptive activity. Because high resorptive activity in vivo is associated with an increase in the average size of osteoclasts in areas of greater resorption and because of the importance of proton extrusion in resorption, we investigated whether the activity of the bafilomycin A(1)-sensitive vacuolar-type H(+)-ATPase (V-ATPase) and amiloride-sensitive Na(+)/H(+) exchanger differed between large and small osteoclasts. Osteoclasts were obtained from newborn rabbit bones, cultured on glass coverslips, and loaded with the pH-sensitive indicator 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Intracellular pH (pH(i)) was recorded in single osteoclasts by monitoring fluorescence. Large (>/=10 nuclei) and small (
Asunto(s)
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoclastos
/
Macrólidos
/
ATPasas de Translocación de Protón Vacuolares
/
Hidrógeno
/
Membranas Intracelulares
Límite:
Animals
Idioma:
En
Revista:
Am J Physiol Cell Physiol
Asunto de la revista:
FISIOLOGIA
Año:
2000
Tipo del documento:
Article
Pais de publicación:
Estados Unidos
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoclastos
/
Macrólidos
/
ATPasas de Translocación de Protón Vacuolares
/
Hidrógeno
/
Membranas Intracelulares
Límite:
Animals
Idioma:
En
Revista:
Am J Physiol Cell Physiol
Asunto de la revista:
FISIOLOGIA
Año:
2000
Tipo del documento:
Article
Pais de publicación:
Estados Unidos