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A genetic screen for novel behavioral mutations in mice.
Sayah, D M; Khan, A H; Gasperoni, T L; Smith, D J.
Afiliación
  • Sayah DM; Department of Molecular and Medical Pharmacology, UCLA School of Medicine, 23-120 CHS, Box 951735, Los Angeles, CA 90095-1735, USA.
Mol Psychiatry ; 5(4): 369-77, 2000 Jul.
Article en En | MEDLINE | ID: mdl-10889547
A genetic screen using mice was performed to identify dominant loci affecting behavior. Mice were mutagenized with ENU, then bred to examine their G1 offspring for behavioral abnormalities. Potentially mutant G1 pups were screened through a variety of behavioral assays, including tests of learning and memory, sensorimotor gating, fear and anxiety, nociception (pain perception) and locomotor activity. Mice falling outside the normal performance distribution in these tests were considered potential behavioral mutants and were bred for further analysis. Outliers included both animals with very discrete defects and animals with abnormal performance across a range of tests. To date, we have identified two confirmed mutants affecting sensorimotor gating. These results provide further impetus for the use of random mutagenesis screens as a tool for dissecting the genetic basis of brain and behavior.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Conducta Animal / Pruebas Genéticas / Umbral del Dolor / Ratones Mutantes Neurológicos / Actividad Motora Límite: Animals Idioma: En Revista: Mol Psychiatry Asunto de la revista: BIOLOGIA MOLECULAR / PSIQUIATRIA Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Conducta Animal / Pruebas Genéticas / Umbral del Dolor / Ratones Mutantes Neurológicos / Actividad Motora Límite: Animals Idioma: En Revista: Mol Psychiatry Asunto de la revista: BIOLOGIA MOLECULAR / PSIQUIATRIA Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido